Contribución al estudio de las mineralizaciones Pb-Zn en el entorno macizo granítico de las Peñas de Haya (Guipúzcoa-Navarra)

Se presenta un primer estudio de las mineralizaciones filonianas PbZn asociadas a pizarras oscuras v granito tardihercínico. Las menas y gangas aparecen fuertemente deformadas y brechificadas y es frecuente la removilización de los sulfuros. En el proceso de formación de estas mineralizaciones se distinguen varias fases: una fase hidrotermal de edad hercínica post-intrusiva y una última fase de removilización. Las paragénesis principales permiten clasificar estos yacimientos como mesotermales. Se discuten los modelos genéticos más probables

Expression of collagenase-3 in the rat ovary during the ovulatory process.

We have examined the expression of the murine counterpart of human collagenase-3, a matrix metalloproteinase produced by breast carcinomas, in the course of processes which involve extensive tissue remodeling. By using Northern blot analysis, we have found that collagenase-3 is expressed in the rat ovary, but not in the remaining analyzed tissues including brain, kidney, liver, lung, mammary gland, uterus, bladder, heart, intestine, prostate, spleen, testis and thymus. Collagenase-3 mRNA was detected at high levels in rat ovaries at proestrus and estrus, was at a minimum at metestrus and started to increase during diestrus through to proestrus. In addition, collagenase-3 was also detected on day 21 of pregnancy, which is approximately one day before parturition. However, no significative expression was detected in RNA from ovaries taken immediately after parturition, or on days 1, 5 or 30 postpartum. Northern blot analysis also revealed that collagenase-3 was not expressed at significant levels, compared with ovarian expression, in the uterus or in the mammary gland during pregnancy or after parturition. When follicular granulosa cells were separated from Northern blot, it was seen that collagenase-3 was not expressed by the granulosa cells but was present in the residual tissue containing interstitial and thecal tissues, growing follicles and corpora lutea. Immunohistochemical studies also confirmed, at the protein level, the localization of collagenase-3 in rat ovary. Gonadotropic stimulation of ovulation in immature rats by priming with pregnant mare's serum gonadotropin and stimulation with human chorionic gonadotropin failed to induce the expression of collagenase-3, suggesting that additional factors which are not present in the immature stimulated rats are needed for completely effective induction of the expression of this matrix metalloproteinase. On the basis of these results, together with the comparative analysis of expression of different matrix metalloproteinases in the rat ovary, we propose that collagenase-3 is a major ovarian metalloproteinase potentially involved in ovarian function during the reproductive cycle.Comisión Interministerial de Ciencia y Tecnología (SAF94-0892

Integration of genetic data on fisheries management models. The European hake case.

The European hake is one of the major commercially important species in Western Europe fisheries. Currently its management is divided into different functional units in the Mediterranean (GFCM, General Fisheries Commission for the Mediterranean) and two stocks in the Atlantic: the Southern and the Northern Stock. The basis for the definition of this management stock structure is under permanent discussion and in the latest years there were an increased amount of genetic studies addressing this topic. Integrated ecosystem assessment requires an improved understanding of the marine ecosystem to provide an effective advice. To this end we have gathered hake genetic information from different studies with the aim of developing a spatial assessment model that links the current stocks. These studies show a restricted gene flow between Southern hake and Mediterranean hake, although a smaller genetic distance between Southern and Northern stocks than one expected from two independent populations, suggesting the presence of a major gene flow from northern grounds core stock (Porcupine and Great Sole Banks) towards the Bay of Biscay and the northwest coast of the Iberian Peninsula. However the quantitative implementation of genetic metrics (such as Fst, migration rates, etc) into population dynamic models is not straightforward given the different nature and assumptions behind these different approaches. In this work we discuss the problems to link these different disciplines suggesting ways to overcome them. These include ways to improve future genetic sampling and developing plausible scenarios for genetic connectivity in population dynamic models

Photorefractive nonlinear propagation of single beams in undoped LiNbO3: Self-defocusing and beam break-up

Beam propagation in photorefractive LiNbO3 planar waveguides has been studied at different beam intensities and propagation lengths. Self-defocusing and beam break-up have been observed and explained using BPM simulations under a 2-centre band transport model

Heavy quark symmetry constraints on semileptonic form factors and decay widths of doubly heavy baryons

We show how heavy quark symmetry constraints on doubly heavy baryon semileptonic decay widths can be used to test the validity of different quark model calculations. The large discrepancies in the results observed between different quark model approaches can be understood in terms of a severe violation of heavy quark spin symmetry constraints by some of those models.Comment: 10 LaTex pages, 3 figures, 6 tables. Corrected and enlarged versio

Test of the heavy quark-light diquark approximation for baryons with a heavy quark

We check a commonly used approximation in which a baryon with a heavy quark is described as a heavy quark-light diquark system. The heavy quark influences the diquark internal motion reducing the average distance between the two light quarks. Besides, we show how the average distance between the heavy quark and any of the light quarks, and that between the heavy quark and the center of mass of the light diquark, are smaller than the distance between the two light quarks, which seems to contradict the heavy quark-light diquark picture. This latter result is in agreement with expectations from QCD sum rules and lattice QCD calculations. Our results also show that the diquark approximations produces larger masses than the ones obtained in a full calculation.Comment: 9 latex pages, 5 figures, 6 table

Self-sufficient asymmetric reduction of β-ketoesters catalysed by a novel and robust thermophilic alcohol dehydrogenase co-immobilised with NADH

β-Hydroxyesters are essential building blocks utilised by the pharmaceutical and food industries in the synthesis of functional products. Beyond the conventional production methods based on chemical catalysis or whole-cell synthesis, the asymmetric reduction of β-ketoesters with cell-free enzymes is gaining relevance. To this end, a novel thermophilic (S)-3-hydroxybutyryl-CoA dehydrogenase from Thermus thermophilus HB27 (Tt27-HBDH) has been expressed, purified and biochemically characterised, determining its substrate specificity towards β-ketoesters and its dependence on NADH as a cofactor. The immobilization of Tt27-HBDH on agarose macroporous beads and its subsequent coating with polyethyleneimine has been found the best strategy to increase the stability and workability of the heterogeneous biocatalyst. Furthermore, we have embedded NADH in the cationic layer attached to the porous surface of the carrier. Since Tt27-HBDH catalyses cofactor recycling through 2-propanol oxidation, we achieve a self-sufficient heterogeneous biocatalyst where NADH is available for the immobilised enzymes but its lixiviation to the reaction bulk is avoided. Taking advantage of the autofluorescence of NADH, we demonstrate the activity of the enzyme towards the immobilised cofactor through single-particle analysis. Finally, we tested the operational stability in the asymmetric reduction of β-ketoesters in batch, succeeding in the reuse of both the enzyme and the co-immobilised cofactor up to 10 reaction cycles

Characterization of IHF Binding to DNA Four-Way Junctions and Forks

The objective of the study is to characterise the mechanical properties of Ti-15Zr binary alloy dental implants and to describe their biomechanical behaviour as well as their osseointegration capacity compared with the conventional Ti-6Al-4V (TAV) alloy implants. The mechanical properties of Ti-15Zr binary alloy were characterised using Roxoli

Integrating spatial management measures into fisheries: The Lepidorhombus spp. case study

Most fisheries management systems rely on a set of regulatory measures to achieve desired objectives. Controls on catch and effort are usually supplemented with gear restrictions, minimum landing sizes, and in the framework of the new common fisheries policy, limitation of discards and by-catch. However, the increasing use of spatial management measures such as conservation areas or spatial and temporal area closures faces new challenges for fishery managers. Here we present an integrated spatial framework to identify areas in which undersized commercial species are more abundant. Once these areas are identified they could be avoided by fishers, minimizing the fishing impact over the immature fraction of the stocks. In particular we applied this methodology to two species of megrim, Lepidorhombus whiffiagonis and L. boscii, in North Atlantic Iberian waters (ICES Divisions 8c and 9a), analyzing fishery-independent data provided by bottom-trawl surveys and environmental data through Bayesian spatial models. Results show that species exhibit species-specific spatial patterns, and we identified sensitive areas that could be used for conservation purposes. We discuss integrating technical measures together (e.g. Minimum Conservation Reference Size and spatial closures) could be a more effective approach for fishery management and this case study could be extended to other species.Postprint1,86